abstract：:New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...

journal_title：Molecular bioSystems

authors： Greco SJ,Rameshwar P

更新日期：2010-02-01 00:00:00

abstract：:Metabolomics is a rapidly growing branch of post-genomic chemical biology. The fission yeast Schizosaccharomyces pombe is an excellent eukaryotic model organism. Although the entire S. pombe genome has been sequenced and detailed transcriptomic analyses were performed, little metabolic profiling has been done. Here we...

journal_title：Molecular bioSystems

authors： Pluskal T,Nakamura T,Villar-Briones A,Yanagida M

更新日期：2010-01-01 00:00:00

abstract：:The identification of a suitable non-fluorescent molecule that can be oxidized by G-quadruplexes into a fluorescent product will be important for several fields such as bioanalyte sensing and cancer therapeutic discovery. Herein, we demonstrate that 2',7'-dichlorodihydrofluorescein diacetate is a superior reducing sub...

journal_title：Molecular bioSystems

authors： Nakayama S,Sintim HO

更新日期：2010-01-01 00:00:00

abstract：:Protein-protein interactions play an essential role in the regulation of various cellular processes. Understanding the recognition mechanism of protein-protein complexes is a challenging task in molecular and computational biology. In this work, we have developed an energy based approach for identifying the binding si...

journal_title：Molecular bioSystems

authors： Gromiha MM,Yokota K,Fukui K

更新日期：2009-12-01 00:00:00

abstract：:Differential gene expression governs the development, function and pathology of multicellular organisms. Transcription regulatory networks study differential gene expression at a systems level by mapping the interactions between regulatory proteins and target genes. While microarray transcription profiles are the most...

journal_title：Molecular bioSystems

authors： Vermeirssen V,Joshi A,Michoel T,Bonnet E,Casneuf T,Van de Peer Y

更新日期：2009-12-01 00:00:00

abstract：:Gene regulatory networks constitute the first layer of the cellular computation for cell adaptation and surveillance. In these webs, a set of causal relations is built up from thousands of interactions between transcription factors and their target genes. The large size of these webs and their entangled nature make it...

journal_title：Molecular bioSystems

authors： Rodríguez-Caso C,Corominas-Murtra B,Solé RV

更新日期：2009-12-01 00:00:00

abstract：:At the intersection between artificial intelligence and statistics, supervised learning allows algorithms to automatically build predictive models from just observations of a system. During the last twenty years, supervised learning has been a tool of choice to analyze the always increasing and complexifying data gene...

journal_title：Molecular bioSystems

authors： Geurts P,Irrthum A,Wehenkel L

更新日期：2009-12-01 00:00:00

abstract：:Information on extracellular signals and conditions is often transduced by biological systems using cascades of protein phosphorylation that affect the activity of enzymes, the localization of proteins and gene expression. A model to study signal transduction is the response of the yeast Saccharomyces cerevisiae to os...

journal_title：Molecular bioSystems

authors： Soufi B,Kelstrup CD,Stoehr G,Fröhlich F,Walther TC,Olsen JV

更新日期：2009-11-01 00:00:00

abstract：:With the advent of new cancer therapies in the last few years, the goals of reducing disease burden and improving quality of life are frequently achieved. Yet despite the advances seen with numerous monotherapies, a multimodality approach that targets different aspects of tumor biology may yield the greatest clinical ...

journal_title：Molecular bioSystems

authors： Kamrava M,Bernstein MB,Camphausen K,Hodge JW

更新日期：2009-11-01 00:00:00

abstract：:A liquid chromatography coupled with mass spectrometry (LC/MS) based metabonomics approach was applied to characterize the aging of rats, and the anti-aging effect of total flavones of Epimedium (TFE), a traditional Chinese medicine, has also been investigated. Serum samples collected from 4, 10, 18 and 24 month-old r...

journal_title：Molecular bioSystems

authors： Yan S,Wu B,Lin Z,Jin H,Huang J,Yang Y,Zhang X,Shen Z,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:Growth factor receptor signaling via receptor tyrosine kinases (RTKs) is one of the basic cellular communication principals found in all metazoans. Extracellular signals are transferred via membrane spanning receptors into the cytoplasm, reversible tyrosine phosphorylation being the hallmark of all RTKs. In recent yea...

journal_title：Molecular bioSystems

authors： Dengjel J,Kratchmarova I,Blagoev B

更新日期：2009-10-01 00:00:00

abstract：:Small molecule modulators are critical for dissecting and understanding signaling pathways at the molecular level. Interleukin 6 (IL-6) is a cytokine that signals via the JAK-STAT pathway and is implicated in cancer and inflammation. To identify modulators of this pathway, we screened a chemical collection against an ...

journal_title：Molecular bioSystems

authors： Johnson RL,Huang R,Jadhav A,Southall N,Wichterman J,MacArthur R,Xia M,Bi K,Printen J,Austin CP,Inglese J

更新日期：2009-09-01 00:00:00

abstract：:We have investigated the thermodynamics of the interaction between the TATA-box-binding protein from Pyrococcus horikoshii (PhoTBP) and its target DNA (TATA-1). The interaction between PhoTBP and double-stranded DNA (dsDNA) is entropically favorable and enthalpically unfavorable. The thermodynamic parameters for TATA-...

journal_title：Molecular bioSystems

authors： Nagatoishi S,Tanaka Y,Kudou M,Tsumoto K

更新日期：2009-09-01 00:00:00

abstract：:The advent of large scale sequencing methods has enabled analyses of the protein-coding parts of cancer genomes to find the mutated genes that cause common human cancers. Unbiased mutation analyses of human tumors originating in the breast, colon, brain, and pancreas have revealed genomic landscapes composed of a few ...

journal_title：Molecular bioSystems

authors： Ali MA,Sjöblom T

更新日期：2009-09-01 00:00:00

abstract：:Drug promiscuity is one of the key issues in current drug development. Many famous drugs have turned out to behave unexpectedly due to their propensity to bind to multiple targets. One of the primary reasons for this promiscuity is that drugs bind to multiple distinctive target environments, a feature that we call mul...

journal_title：Molecular bioSystems

authors： Park K,Lee S,Ahn HS,Kim D

更新日期：2009-08-01 00:00:00

abstract：:Mirror image phage display is a straightforward approach to identify new potentially therapeutically active D-enantiomeric peptides. Such D-peptides are more resistant to proteolytic degradation compared to L-peptides. In this review, several examples of mirror image phage display derived D-peptides with therapeutical...

journal_title：Molecular bioSystems

authors： Funke SA,Willbold D

更新日期：2009-08-01 00:00:00

abstract：:The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potent...

journal_title：Molecular bioSystems

authors： Tian J,Ma K,Saaem I

更新日期：2009-07-01 00:00:00

abstract：:Low transfection efficiency and inactivation by serum are the major drawbacks for cationic polymers when used as non-viral gene vectors. Here, a series of N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI) copolymers with different compositions were synthesized through grafting low molecular weight PEI (800 Da) to...

journal_title：Molecular bioSystems

authors： Lu B,Sun YX,Li YQ,Zhang XZ,Zhuo RX

更新日期：2009-06-01 00:00:00

abstract：:The study of mitochondria and mitochondrial Ca2+ signalling in localised regions is hampered by the lack of tools that can uncouple the mitochondrial membrane potential (DeltaPsi(m)) in a spatially predefined manner. Although there are a number of existing mitochondrial uncouplers, these compounds are necessarily memb...

journal_title：Molecular bioSystems

authors： Avlonitis N,Chalmers S,McDougall C,Stanton-Humphreys MN,Brown CT,McCarron JG,Conway SJ

更新日期：2009-05-01 00:00:00

abstract：:A growing number of diverse proteins are being identified that moonlight. Moonlighting proteins comprise an interesting subset of multifunctional proteins in which the two functions are found in a single polypeptide chain. They do not include proteins that are multifunctional due to gene fusions, families of homologou...

journal_title：Molecular bioSystems

authors： Jeffery CJ

更新日期：2009-04-01 00:00:00

abstract：:A metabolomic study was performed to investigate the biochemical profiles of livers from male and female zebrafish (Danio rerio), using a multiple platform approach, incorporating 1H NMR, GC/MS and LC/MS. The reproducibility and reliability of the three methods were validated prior to the assays. Major biomolecules de...

journal_title：Molecular bioSystems

authors： Ong ES,Chor CF,Zou L,Ong CN

更新日期：2009-03-01 00:00:00

abstract：:The significant increase in the demand for purified protein for crystallization and structural studies has made necessary the development of multi-sample methods for identifying solution conditions that affect protein stability and aggregation. Conditions that stabilize proteins can improve protein purification and cr...

journal_title：Molecular bioSystems

authors： Senisterra GA,Finerty PJ Jr

更新日期：2009-03-01 00:00:00

abstract：:The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel. ...

journal_title：Molecular bioSystems

authors： Kim J,Kwon D,Lee J,Pasquier H,Grailhe R

更新日期：2009-02-01 00:00:00

abstract：:Cyclic adenosine monophosphate and cyclic guanosine monophosphate are universal intracellular messengers whose concentrations are regulated by molecular networks comprised of different isoforms of the synthases adenylate cyclase or guanylate cyclase and the phosphodiesterases which degrade these compounds. In this pap...

journal_title：Molecular bioSystems

authors： Valeyev NV,Heslop-Harrison P,Postlethwaite I,Gizatullina AN,Kotov NV,Bates DG

更新日期：2009-01-01 00:00:00

abstract：:Many Gram-negative bacterial pathogens use type III secretion systems (TTSSs) for subverting the normal cellular functions of their target eukaryotic cells. The type III secretion apparatus (TTSA) functions like a syringe to inject proteins through an external needle and into a target cell's membrane and cytosol. The ...

journal_title：Molecular bioSystems

authors： Wang Y,Zhang L,Picking WL,Picking WD,De Guzman RN

更新日期：2008-12-01 00:00:00

abstract：:Over the last few years important new insights into the process of long-range gene regulation have been obtained. Gene regulatory elements are found to engage in direct physical interactions with distant target genes and with loci on other chromosomes to modulate transcription. An overview of recently discovered long-...

journal_title：Molecular bioSystems

authors： Miele A,Dekker J

更新日期：2008-11-01 00:00:00

abstract：:Cells of the bacterium Myxococcus xanthus organize into two types of patterns depending on their nutritional status, i.e. in the presence of nutrients cells form spreading colonies and in the absence of nutrients cells form fruiting bodies. Formation of both patterns depends on directed cell movements, which, in turn,...

journal_title：Molecular bioSystems

authors： Leonardy S,Bulyha I,Søgaard-Andersen L

更新日期：2008-10-01 00:00:00

abstract：:Protein phosphorylation is associated with most cell signaling and developmental processes in eukaryotes. Despite the vast extent of the phosphoproteome within the cell, connecting specific kinases with relevant targets remains a significant experimental frontier. The challenge of linking kinases and their substrates ...

journal_title：Molecular bioSystems

authors： Sopko R,Andrews BJ

更新日期：2008-09-01 00:00:00

abstract：:Plant stress hormones, such as jasmonates (JAs) and ethylene (ET) are essential in plant defence against stress conditions. JAs are used in cosmetics and food flavouring, and the recently demonstrated anti-cancer activity of JAs highlights their potential in health protection. It reinforces the need for a better under...

journal_title：Molecular bioSystems

authors： Schweighofer A,Meskiene I

更新日期：2008-08-01 00:00:00

abstract：:Stochastic fluctuations (noise) in gene expression can cause members of otherwise genetically identical populations to display drastically different phenotypes. An understanding of the sources of noise and the strategies cells employ to function reliably despite noise is proving to be increasingly important in describ...

journal_title：Molecular bioSystems

authors： Tabor JJ,Bayer TS,Simpson ZB,Levy M,Ellington AD

更新日期：2008-07-01 00:00:00

abstract：:Bacterial biofilms are defined as a community of surface-attached bacteria that are protected by an extracellular matrix of biomolecules. We have recently reported the synthesis of a small molecule, denoted TAGE, based on the natural product bromoageliferin and demonstrated that TAGE has anti-biofilm activity against ...

journal_title：Molecular bioSystems

authors： Huigens RW 3rd,Ma L,Gambino C,Moeller PD,Basso A,Cavanagh J,Wozniak DJ,Melander C

更新日期：2008-06-01 00:00:00

abstract：:A novel class of pH-sensitive PEG lipids bearing acid-cleavable acetal linkages and short PEG chains have been synthesised and used in ternary vector formulations. The cleavage pH was influenced by structural components including the terminal PEG moiety and spacer length. ...

journal_title：Molecular bioSystems

authors： Wong JB,Grosse S,Tabor AB,Hart SL,Hailes HC

更新日期：2008-06-01 00:00:00

abstract：:Transient and low-affinity interactions among macromolecules underlie many physiological events. Often, these interactions are difficult to study because they are not maintained when the participating molecules are removed from their cellular context. To circumvent this challenge, crosslinking reagents can be used to ...

journal_title：Molecular bioSystems

authors： Tanaka Y,Bond MR,Kohler JJ

更新日期：2008-06-01 00:00:00

abstract：:Structural insight into DNA quadruplex structures formed by oligodeoxyribonucleotides 3'TG5'-5'GGGT3' (QS55) and 5'TG3'-3'GGGT5' (QS33) is presented. NMR analysis reveals that QS33 forms a parallel-like four-fold symmetric quadruplex, while QS55 possesses a two-fold symmetry and is characterized by a tetrameric antipa...

journal_title：Molecular bioSystems

authors： Galeone A,Mayol L,Virgilio A,Virno A,Randazzo A

更新日期：2008-05-01 00:00:00

abstract：:Primarily used for medicinal purposes in the past, biologically active small molecules have been increasingly employed to explore complex biological processes in the era of "chemical genetics". Since the contributions of this small molecule approach to biology have been extensive, we limit the focus of our review to t...

journal_title：Molecular bioSystems

authors： Wehenkel M,Hong JT,Kim KB

更新日期：2008-04-01 00:00:00

abstract：:We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired (1)H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6...

journal_title：Molecular bioSystems

authors： Wagstaff JL,Sadovskaya I,Vinogradov E,Jabbouri S,Howard MJ

更新日期：2008-02-01 00:00:00

abstract：:Systematic screens for human disease genes have emerged in recent years, due to the wealth of information provided by genome sequences and large scale datasets. Here we review how integration of genomic data in yeast and human is helping to elucidate the genetic basis of mitochondrial diseases. The identification of n...

journal_title：Molecular bioSystems

authors： Perocchi F,Mancera E,Steinmetz LM

更新日期：2008-01-01 00:00:00

abstract：:In the field of synthetic biology, recent genetic engineering efforts have enabled the construction of novel genetic circuits with diverse functionalities and unique activation mechanisms. Because of these advances, artificial genetic networks are becoming increasingly complex, and are demonstrating more robust behavi...

journal_title：Molecular bioSystems

authors： Sayut DJ,Kambam PK,Sun L

更新日期：2007-12-01 00:00:00

abstract：:The neuroscientific community rapidly adopted RNA interference techniques as an experimental tool for the dissection of gene function in vitro and in animal models of neurological disease in vivo. Here, we discuss recent advances in the biotechnical implementation of siRNA/shRNA-mediated gene silencing focusing on iss...

journal_title：Molecular bioSystems

authors： Lingor P,Bähr M

更新日期：2007-11-01 00:00:00

abstract：:In this review we bring together recent results from our group focused towards the development of biosensors from single conically-shaped artificial nanopores. The nanopores, used in the work presented here, were prepared using the track-etch process. The fabrication of track-etched conical nanopores has been optimize...

journal_title：Molecular bioSystems

authors： Sexton LT,Horne LP,Martin CR

更新日期：2007-10-01 00:00:00